The “Multi-scale trait-based approach to link biological structure to ecosystem function” tool (Multi-scale TBA tool) was developed to i) advance trait-based approaches across taxonomic (e.g. benthic organisms, fish, phytoplankton) and organisational scales (e.g. species, communities, habitats, seascapes-), and to ii) provide step-wise guidance, concrete examples and code of analysis. By providing insights into spatial representations (maps) of functioning (traits) and functional diversity it may support, for example, the identification of functionally important areas, and informing marine spatial planning and management in coastal areas regarding functional patterns.