
Category: Ecological
Subcategory: Ecosystem functioning & biodiversity
Tool Type : Equipment & protocol
Input data: Biological samples
Output: Pictures, measurements
Target users: Researchers
Location tested: Gulf of Heraklion
Level of uncertainty: N/A
- Version: The equipment itself does not have a version. The dataset derived from the MARBEFES project is Version 1.
- Publication: Vernadou E., Keklikoglou K., Chatzinikolaou E., Anastasiadis A., Grigoriou P., Dailianis T., 2025: Micro-CT morphological traits for two invertebrate species in 2023. Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Center for Marine Research. https://marineinfo.org/doc/dataset/8885
- Rights: This dataset is licensed under a Creative Commons Attribution 4.0 International License.
- Difficulty level of implementation: Complex
- Skill required (for method use): Well-trained personnel are required for operating the micro-CT system and performing 3D analyses
- Authors: Vernadou E., Keklikoglou Kleoniki, Chatzinikolaou Evangelia, Anastasiadis Athanasios, Grigoriou Panagiotis, Dailianis Athanasios
- Tool contributors: Vernadou Emmanouela, Chatzinikolaou Evangelia, Keklikoglou Kleoniki
- Project general coordinator: Panagiotis Kasapidis
- Vernadou E., Keklikoglou K, Chatzinikolaou E., Anastasiadis A., Grigoriou P., Dailianis T., 2025: Micro-CT morphological traits for two invertebrate species in 2023. Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Center for Marine Research. https://marineinfo.org/doc/dataset/8885
- https://www.vliz.be/en/imis?module=dataset&dasid=8885
- MicroCT vlab (https://microct.portal.lifewatchgreece.eu/) A virtual laboratory which was created in the framework of the LifeWatchGreece project (https://www.lifewatchgreece.eu/) and offers virtual galleries and online tools for the 3D manipulation of the micro-CT datasets.
- Chatzinikolaou, E. et al. (2016). ICES Journal of Marine Science – DOI: 1093/icesjms/fsw219
- Keklikoglou, K. et al. (2019). European Journal of Taxonomy – DOI: 5852/ejt.2019.522
- Chatzinikolaou, E. et al. (2021). Frontiers in Marine Science – DOI: 3389/fmars.2021.645660
- Name: Kleoniki Keklikoglou
- Organization: Hellenic Centre for Marine Research (HCMR)
- Email: keklikoglou@hcmr.gr
Micro-CT
Micro-CT is an X-ray imaging technique that enables the visualization of both internal and external structures of a sample in three dimensions. The technique is based on generating a series of projection images by rotating a specimen positioned between an X-ray source and a detector. Owing to its non-destructive nature, micro-CT has become an essential tool in taxonomic research, as it reveals taxonomically important internal structures in their original spatial context. Beyond taxonomy, micro-CT datasets allow for advanced 3D morphological analyses, including measurements of density, porosity, and structural thickness. Such quantitative traits can be proposed as novel numerical trait data for marine invertebrates, helping to address the gaps in the existing morphological data repositories and relevant literature. Moreover, these traits may also serve as “response traits,” as they can reflect organismal response to environmental shifts induced by anthropogenic stressors such as climate change and ocean acidification. For instance, density and structural thickness of calcified structures can provide valuable insights into calcification processes and reef-building functions, while 3D porosity analysis can inform estimates of the filtration capacity in marine invertebrates such as sponges.